Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat O-6-Methylguanine DNA Methyltransferase (also known as MGMT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MGMT antibody. MGMT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MGMT Antibody is added and binds to MGMT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MGMT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MGMT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm